Changes in serum markers of oxidative status in Wistar rats following exposure to kerosene

Objective: Kerosene is commonly available in many parts of the developing world where it is used as fuel for lighting and cooking purposes. Toxic effects of large quantities of kerosene have been reported but there is dearth of data on impact of trace amount of kerosene on mammals. Exposure through either the dermal or oral route is common among humans (kerosene retailers). The aim of the study is to determine the degree of oxidative damage that can result from dermal or oral exposure to trace amount of kerosene. This will be accomplished by utilizing oxidative-stress markers like antioxidant enzymes and product of lipid peroxidation as indices of the study. Methods: Using spectrometric techniques; levels of reduced glutathione (GSH), oxidized glutathione (GSSG) and malondialdehyde (MDA) as well as activities of glutathione reductase, glutathione-S-transferase, catalase, superoxide dismutase and glutathione peroxidase were measured. Results: Administration of rats with 0.4 ml/kg b.wt of kerosene for a period of thirty days resulted in significant decreases in either the level or activities of antioxidants (Zn, Cu, Mn, GSH, antioxidant enzymes) and significant increases in the levels of products of oxidation (MDA, GSSG). Conclusion: The result of this study suggests that constant exposure to trace quantity of kerosene may be capable of inducing oxidative stress in mammals. Therefore steps should be taken to discourage constant exposure to kerosene.